anti p2x7r  (Alomone Labs)

Journal: Biomolecules

Article Title: Type II Cells in the Human Carotid Body Display P2X7 Receptor and Pannexin-1 Immunoreactivity

doi: 10.3390/biom15111523

Figure Lengend Snippet: Immunofluorescence-based detection of P2X7r in the human carotid body (CB), petrosal ganglia (PG) and cervical sympathetic ganglia (SG). Within the CB, P2X7r was detected in cells whose morphology and arrangement suggest they are type II cells ( a , b ). In the PG ( c ) and SG ( d ), intense immunofluorescence for P2X7r was observed in the satellite glial cells (white arrows) and in a subpopulation of SG neurons; in the PG, weak immunofluorescence for P2X7r was detected in some neuronal bodies. n: neuron bodies. Scale bar is the same for ( a , b ). Objective: 20×/0.75 oil; pinhole: 1; XY resolution: 156 nm; and Z resolution: 334 nm.

Article Snippet: P2X7R , Rabbit , 1:400 , Alomone Labs 1.

Techniques: Immunofluorescence

Journal: Biomolecules

Article Title: Type II Cells in the Human Carotid Body Display P2X7 Receptor and Pannexin-1 Immunoreactivity

doi: 10.3390/biom15111523

Figure Lengend Snippet: Immunofluorescence-based detection of P2X7r ( a – d ) and pannexin 1 ( e – h ) in the human carotid body. Cells displaying P2X7r immunofluorescence (green fluorescence in ( a )) and pannexin 1 immunofluorescence (green fluorescence in ( e )) form a dense meshwork throughout glomic glomeruli consistent with their presence in type II cells. Type I cells showed strong immunofluorescence for SYN (red fluorescence in ( b , f )). Merger (yellow fluorescence) of P2X7r ( c , d ) or pannexin 1 ( g , h ) with SYN, showing cell segregation. The occasional merger of P2X7r and pannexin 1 and SYN was found in disperse drops (yellow arrows). Scale bar is the same for ( a – c , e – g ); scale bar is the same for ( d , h ). Objective: 20×/0.75 oil; pinhole: 1; XY resolution: 156 nm; and Z resolution: 334 nm.

Article Snippet: P2X7R , Rabbit , 1:400 , Alomone Labs 1.

Techniques: Immunofluorescence, Fluorescence

Journal: Biomolecules

Article Title: Type II Cells in the Human Carotid Body Display P2X7 Receptor and Pannexin-1 Immunoreactivity

doi: 10.3390/biom15111523

Figure Lengend Snippet: Immunofluorescence-based detection of P2X7r ( a – d ) and pannexin 1 ( e – h ) in the human carotid body. Cells displaying P2X7r immunofluorescence (green fluorescence in ( a )) and pannexin 1 immunofluorescence (green fluorescence in ( e )) as well as S100 protein immunofluorescence (S100P; red fluorescence in ( b , f )) form a dense meshwork throughout glomic glomeruli consistent with their presence in type II cells. The merger (yellow fluorescence) of P2X7r ( c , d ) or pannexin 1 ( g , h ) with S100P show cell colocalization of P2X7r and pannexin 1 with S100P, thus confirming that both are localized in type II cells. Scale bar is the same for ( a – c , e – g ); scale bar is the same for ( d , h ). Objective: 20×/0.75 oil; oil; pinhole: 1; XY resolution: 156 nm; and Z resolution: 334 nm.

Article Snippet: P2X7R , Rabbit , 1:400 , Alomone Labs 1.

Techniques: Immunofluorescence, Fluorescence

Journal: Biomolecules

Article Title: Type II Cells in the Human Carotid Body Display P2X7 Receptor and Pannexin-1 Immunoreactivity

doi: 10.3390/biom15111523

Figure Lengend Snippet: Immunofluorescence-based detection of P2X7r ( a – d ) and pannexin 1 ( e – h ) in the human carotid body. P2X7r (green fluorescence in ( a )) and pannexin 1 (green fluorescence in ( e )) within the CB are present in a cell meshwork consistent with its presence in type II cells. Nerve fibre terminal profiles scattered through the CB display immunofluorescence for neurofilament protein (NFP; red fluorescence in ( b , f )). Merge (yellow fluorescence) of P2X7r ( c , d ) and pannexin 1 ( g , h ) with NFP show the presence of both proteins in some nerve fibre terminal profiles (yellow arrows). Scale bar is the same for ( a – c , e – g ); scale bar is the same for ( d , h ). Objective: 20×/0.75 oil; oil; pinhole: 1; XY resolution: 156 nm; and Z resolution: 334 nm.

Article Snippet: P2X7R , Rabbit , 1:400 , Alomone Labs 1.

Techniques: Immunofluorescence, Fluorescence

Journal: Biomolecules

Article Title: Type II Cells in the Human Carotid Body Display P2X7 Receptor and Pannexin-1 Immunoreactivity

doi: 10.3390/biom15111523

Figure Lengend Snippet: Percentage of area occupied by P2X7r and pannexin 1 in the carotid body in type II glomus cells (green) and nerve fibre terminals (yellow). Immunofluorescence was also detected in cells other than type I and type II cells (see also and ). Type I glomus cells lacked both P2X7r and pannexin 1. This diagram completes and shows the distribution of immunofluorescence for P2X7r and pannexin 1 in a relatively manner.

Article Snippet: P2X7R , Rabbit , 1:400 , Alomone Labs 1.

Techniques: Immunofluorescence

Journal: Biomolecules

Article Title: Type II Cells in the Human Carotid Body Display P2X7 Receptor and Pannexin-1 Immunoreactivity

doi: 10.3390/biom15111523

Figure Lengend Snippet: Immunofluorescence-based detection of P2X7r in the petrosal ( a – c ) and cervical sympathetic ganglia ( d – f ). In both petrosal and cervical sympathetic ganglia P2X7r, immunofluorescence (green fluorescence in ( a ) and ( d ), respectively) was detected in satellite glial cells (S100 protein positive; red fluorescence in ( b , e )). The merger (purple fluorescence; white arrows) of P2X7r and S100P shows co-localization of both proteins. P2X7r was also detected in a subpopulation of sensory and sympathetic neurons. n: neurons. Scale bar are identical for ( a – c ) and for ( d – f ). Objective: 40×/1.30 oil; pinhole: 1; XY resolution: 156 nm; and Z resolution: 334 nm.

Article Snippet: P2X7R , Rabbit , 1:400 , Alomone Labs 1.

Techniques: Immunofluorescence, Fluorescence

Journal: Biomolecules

Article Title: Type II Cells in the Human Carotid Body Display P2X7 Receptor and Pannexin-1 Immunoreactivity

doi: 10.3390/biom15111523

Figure Lengend Snippet: Schematic representation of ATP-mediated neurotransmission–gliotransmission. Type I cells release ATP that acts on P2X2 and P2X3 receptors on afferent terminals, and on P2Y1, P2Y2, and P2X7 receptors on type II cells. In turn, the pannexin 1 (PNX1) channels present in type II cells would release more ATP from these cells. The presence of P2X7 and pannexin 1 in the afferent and efferent terminals should be taken with caution pending physiological and pharmacological studies.

Article Snippet: P2X7R , Rabbit , 1:400 , Alomone Labs 1.

Techniques: